Cyclic (1.RAR.2)-.BETA.-D-glucan-hydrolyzing enzymes from Acremonium sp. 15 purification and some properties of endo-(1.RAR.2)-.BETA.-D-glucanase and .BETA.-D-glucosidase.
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منابع مشابه
Purification and Characterization of an Endo-(1,3)-beta-d-Glucanase from Trichoderma longibrachiatum.
A laminarinase [endo-(1,3)-beta-d-glucanase] has been purified from Trichoderma longibrachiatum cultivated with d-glucose as the growth substrate. The enzyme was found to hydrolyze laminarin to oligosaccharides varying in size from glucose to pentaose and to lesser amounts of larger oligosaccharides. The enzyme was unable to cleave laminaribiose but hydrolyzed triose to laminaribiose and glucos...
متن کاملBarley beta-D-glucan exohydrolases with beta-D-glucosidase activity. Purification, characterization, and determination of primary structure from a cDNA clone.
Two beta-glucan exohydrolases of apparent molecular masses 69,000 and 71,000 Da have been purified from extracts of 8-day germinated barley grains and are designated isoenzymes ExoI and ExoII, respectively. The sequences of their first 52 NH2-terminal amino acids show 64% positional identity. Both enzymes hydrolyze the (1,3)-beta-glucan, laminarin, but also hydrolyze (1,3;1,4)-beta-glucan and 4...
متن کاملMolecular cloning of endo-beta-D-1,4-glucanase genes, rce1, rce2, and rce3, from Rhizopus oryzae.
Three endoglucanase genes, designated the rce1, rce2, and rce3 genes, were isolated from Rhizopus oryzae as the first cellulase genes from the subdivision ZYGOMYCOTA: All the amino acid sequences deduced from the rce1, rce2, and rce3 genes consisted of three distinct domains: cellulose binding domains, linker domains, and catalytic domains belonging to glycosyl hydrolase family 45. The rce3 gen...
متن کاملMedical Summary on Beta 1,3-D Glucan
THIS PAGE IS FOR EDUCATIONAL PURPOSES ONLY The information contained on this page is intended to provide you with a balanced overview of information relating to health and well-being. The articles are intended for general education purposes and not to sell any specific product. This page does not constitute labeling, endorsements or advertisements for any particular products and should not be i...
متن کاملAn Arabidopsis endo-1,4-beta-D-glucanase involved in cellulose synthesis undergoes regulated intracellular cycling.
The synthesis of cellulose microfibrils requires the presence of a membrane-bound endo-1,4-beta-D-glucanase, KORRIGAN1 (KOR1). Although the exact biochemical role of KOR1 in cellulose synthesis is unknown, we used the protein as a marker to explore the potential involvement of subcellular transport processes in cellulose synthesis. Using immunofluorescence and a green fluorescent protein (GFP)-...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1987
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.51.2701